Tissue-type Plasminogen Activator (t-PA)
Tissue-Plasminogen Activator (t-PA) is a protein involved in the breakdown of blood clots. It is a serine protease found on endothelial cells, the cells that line the blood vessels. As an enzyme, it catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown. Because it works on the clotting system, tPA is used in clinical medicine to treat embolic or thrombotic stroke. Use is contraindicated in hemorrhagic stroke and head trauma.
TECHNOZYM® t-PA EDTA ELISA Kit
A highly sensitive sandwich ELISA for the quantitative determination of t-PA in human plasma. Suitable also for monitoring t-PA levels during thrombolytic therapy. The assay is a sandwich ELISA employing two monoclonal antibodies. The system detects both complexed and uncomplexed t-PA. The detection limit is 1 ng/mL. The assay is standardized against an International standard. Assay time is 2.5 hours. Kit contents include calibrators, high and low controls, coated plate, peroxidase-labeled antibody, buffers and ready to use TMB substrate. The sample buffer contains EDTA for increased recovery of t-PA in in normal samples.
TECHNOZYM® t-PA Combi Actibind® ELISA Kit
A highly sensitive ELISA system for the combined quantitative determination of antigen concentration and activity of t-PA in human plasma. TECHNOZYM® t-PA Combi Actibind® ELISA Kit employs a capture antibody which does not interfere with t-PA functional activity. Following the binding of t-PA contained in the sample by this antibody, t-PA functional activity is determined using chromogenic plasmin substrate. After measuring the functional activity, bound t-PA antigen is detected using a peroxidase-labeled monoclonal anti t-PA antibody which recognizes both complexed and uncomplexed t-PA.