Plasminogen Activator Inhibitor-Type1 (PAI-1)

Plasminogen Activator Inhibitor-Type-1 (PAI-1) also known as endothelial plasminogen activator    inhibitor or serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. PAI-1 is a serine protease inhibitor (serpin) that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis. PAI-1, is the main inhibitor of the plasminogen activators. PAI-1 inhibits the serine proteases tPA and uPA/urokinase, and hence is an inhibitor of fibrinolysis, the physiological process that degrades blood clots.

TECHNOZYM^® PAI-1 Antigen ELISA Kit

The assay is a double sandwich ELISA employing two monoclonal antibodies. The system detects active PAI-1, latent and t-PA - PAI-1 complexes. The detection limit is 0.5 ng/mL. Assay time is 2.5 hours.

TECHNOZYM^® PAI-1 Actibind^® ELISA Kit

A highly sensitive ELISA System for the quantitative determination of active PAI-1 antigen in human plasma. The kit allows determination of active PAI-1 antigen in plasma of patients also with disseminated intravascular coagulation and in atherosclerotic disease. The Kit is based on the immobilization of functionally active t-PA to plates by means of a monoclonal antibody. PAI-1 contained in the test samples binds to t-PA and is then quantified using a peroxidase-labeled monoclonal anti-PAI-1 antibody. The assay recognizes PAI-1 active form with no interference of PAI-2 (5 U/mL) and PAI-3 (5.5 µg/mL). Assay time is only 60 minutes.

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