Research Products

Plasminogen

Plasminogen, the precursor of the serine protease plasmin, is a single chain glycoprotein with a molecular weight of approximately 92kD. Plasminogen activators cleave a single peptide bond in plasminogen between Arg-560 - Val-561 to generate the two-chain molecule plasmin which is a potent proteolytic enzyme with trypsin-like specificity.

The fibrin specificity of the proteolytic action of the active site contained in the B-chain of plasmin is  mediated by kringle structures in the A-chain which can bind to lysine residues as they are exposed during fibrin formation. These kringle structures are also responsible for the affinity of plasmin(ogen) to alpha-2-antiplasmin, histidin-rich-glycoprotein and thrombospondin.

Plasminogen circulates in plasma in its native form Glu-plasminogen. The limited proteolytic action of plasmin on Glu-plasminogen cleaves a 76 or 67 amino acid aminoterminal peptide generating Lys-or Arg-plasminogen, respectively. Lys-plasminogen has higher affinity for lysine residues and is more readily activated by plasminogen activator than Glu-plasminogen.

The functional domains of plasminogen are revealed by elastase cleavage of the molecule which result in 3 major fragments: kringle 1-3 fragment, kringle 4 fragment and kringle 5 containing mini-plasminogen.

Monoclonal Antibody

TC 1PG

TC 2PG

TC
4PG

TC 7PG

Class

IgG1

IgG1

IgG1

IgG1

Binding to plasminogen

Glu-Plg

K1-3

Mini-
Plg

K4

Binding to plasmin-alpha-2-antiplasmin complex

+

+

-

+

Half-max. binding (ng/ml)

0.006

0.010

n.d.

n.d.

Inhibition of plasminogen activation

-

-

Competitive
Ki=4nM

-

Stimulation of plasminogen activation

+

+

-

+

Glu-Plasminogen protein

Purified from human plasma, lyophilized

REF Package Size MSDS
TC41004 1 mg Download MSDS
TC41005 5 mg Download MSDS

Lys-Plasminogen protein

Purified from human plasma, lyophilized

REF Package Size MSDS
TC41014 1 mg Download MSDS
TC41015 5 mg Download MSDS

Monoclonal anti plasminogen antibody 1PG

Lyophilized, IgG1

REF Package Size MSDS Insert
TC21103 500 µg Download MSDS Download Insert

Monoclonal anti plasminogen antibody 2PG

Lyophilized, IgG1

REF Package Size MSDS Insert
TC21113 500 µg Download MSDS Download Insert

Monoclonal anti plasminogen antibody 4PG

Lyophilized, IgG1

REF Package Size MSDS Insert
TC21123 500 µg Download MSDS Download Insert

Monoclonal anti plasminogen antibody 7PG

Lyophilized, IgG1

REF Package Size MSDS Insert
TC21133 500 µg Download MSDS Download Insert