Thrombophilia

APC Control Kit [CE]

Control plasma for verification of FV Leiden mutation (FV:Q506) in assays determining the functional activity of activated protein C to inactivate FV (APC resistance caused by the FV Leiden mutation). Application sheets for analyzers are available on request.

Kit content:

1 x FV-L Negative Controls
1 x FV-L Heterozygous Controls

APC Resistance Kit [CE]

The APC Resistance Kit is a clotting assay for determination of APC resistance caused by the FV Leiden mutation. It is based on a F V dependent prothrombin activator isolated from snake venom. The APC Resistance Kit is a functional assay that differs from other functional APC resistance tests as it acts on the level of the prothrombinase complex. Robustness of the assay is achieved by eliminating possible interfering influences from factors down-stream of the prothrombinase complex and its independency from added calcium. Application sheets for analyzers are available on request.

Kit content for ~120 tests/~40 tests:

3/1 x APC/RVV-V (+APC) Reagent
3/1 x RVV-V (-APC) Reagent
3/1 x PTA Reagent
3/1 x Dilution plasma
1 x FV-L Negative Control
1 x FV-L Heterozygous Control

Genotyping Kit - Venous Thrombosis

The Genotyping Kit ? Venous Thrombosis allows the determination of the R/Q 506 mutation in the factor V gene (Factor V Leiden), the 20210 G/A mutation in the prothrombin (Factor II) gene and A/V 223 (677 C/T) mutation in the 5,10-methylene-tetrahydrofolate reductase gene (MTHFR).

The assay consists of three steps:

1. DNA extraction
2. DNA amplification by PCR
3. analysis of PCR products by gel-electrophoresis.

Step (2) is based on a "multiplex" PCR reaction, which combines multiple primer combinations in the same reaction tube. In this kit 6 specific primer combinations are used to amplify mutant as well as wild type (normal variant) alleles of three different genes. Thus, homozygous and heterozygous carriers of the mutations can be identified by one PCR test. The Genotyping Kit contains DNA diluent (sterile 2x3 ml), mastermix for 2x11 PCR reactions(2x360 µl), size marker (1x20 µl), Sybr®Green I (5µl sample), 2 ready to use Spreadex® EL 400 Mini gels, 1 gel frame and the Electrophoresis buffer 40 x concentrated.

Sybr®Green is a trademark of Molecular Probes, Inc.,USA

Spreadex® is a trademark of Elchrom Scientific AG