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TECHNOZYM® PAI-1 Actibind® ELISA Kit [CE]
A high sensitive complete sandwich ELISA for the quantitative determination of active PAI-1 antigen in human plasma. The kit allows determination of active PAI-1 antigen in plasma of patients also with disseminated intravascular coagulation and in atheriosclerotic disease. The Plasminogen Activator Inhibitor type 1 (PAI-1) Actibind® ELISA Kit is based on the immobilization of functionally active t-PA to plates by means of a monoclonal antibody. PAI-1 contained in the test samples binds to t-PA and is then quantified using a peroxidase-labeled monoclonal anti-PAI-1 antibody. The assay recognizes PAI-1 active form with no interference from PAI-2 (5 U/mL) and PAI-3 (5.5 µg/mL). Acidified citrated plasma, citrated, CTAD or EDTA plasmas can be used. The assay is standardized with International standard 87/512. Assay time is only 60 minutes.
Kit contents include standards, high and low controls, coated plate, peroxidase-labeled antibody, buffers and ready to use TMB substrate.
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